EZHFGF23

Human FGF-23 ELISA Kit

measuress and quantifies FGF-23 levels in 50 μL serum, plasma or cell culture supernatent

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

Properties

• product name: Human FGF-23 ELISA Kit, This Human FGF-23 ELISA Kit is used to measure & quantify FGF-23 levels in Metabolism & Inflammation & Immunology research.
• Quality Level: 200
• species reactivity: human
• packaging: kit of 1 × 96 wells
• parameter: 50 μL sample volume (4hr assay)
• assay range: sensitivity: 9.9 pg/mL; (minimum detectable concentrations in tissue culture/serum/plasma); standard curve range: 9.9-2400 pg/mL; inter-assay cv: <11%; intra-assay cv: <11%
• technique(s): ELISA: suitable
• input: sample type cell culture supernatant; sample type serum; sample type plasma (K2 EDTA)
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Studies demonstrate that Fibroblast Growth Hormone-23 plays a critical role as a phosphate-regulating hormone. FGF-23 is responsible for the inhibition of renal tubular reabsorption of inorganic phosphorus and, consequently, the regulation of phosphorus homeostasis. FGF-23 mRNA is expressed in the brain, in the thymus at low levels, in dendritic cells, with the highest expression in osteocytes and osteoblasts during phases of active bone remodeling. Because circulating levels of FGF-23 are regulated by vitamin D and phosphorus, normal skeletal and soft tissue development require vitamin D and a functional vitamin D signaling pathway. Diseases resulting from aberrant FGF-23 expression include inherited forms of rickets, certain kidney diseases and phosphorus-related tumors.

Specificity

The antibody pair used in this assay cross-reacts 0.01% to Human FGF- 21 and 0.01% to FGF-19.

No significant cross-reactivity has been observed to other human and rat molecules/hormones tested

Application

50µl sample size, 2-Step assay with <4hr incubation time.

Research Category
Stem Cell Research

Used to detect/quantify: FGF-23

Other Notes

Please contact Technical Service for linearity of dilution.

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS05,GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H290 - H302 + H332 - H311 - H317 - H411
• Precautionary Statements: P234 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3