AB5471P
Anti-Semaphorin 3F Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SEMA3F(6405)
Specificity
Recognizes Semaphorin 3F (Sema3F), recombinant and purified protein. By Western blot the antibody recognizes a protein of 90 kDa in total extracts from tissue culture cells producing Sema3F. Semaphorin 3F is expressed ubiquitously with higher levels in lung, brain, spleen, muscle and heart.
This antibody may also react with mouse because of extremely high sequence conservation in the SEM3F protein.
Immunogen
Synthetic peptide from human Semaphorin 3F (Catalog number AG363). (TPWTQTQAVRFRFSRATDGALRPM).
Application
Anti-Semaphorin 3F Antibody is an antibody against Semaphorin 3F for use in IC & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Growth Cones & Axon Guidance
Western blot: 0.5-1.0 μg/mL using ECL. Suggested antibody dilution buffer is TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl with 0.05% Tween 20). Suggested incubation is for two hours at room temperature or overnight at 2-8°C.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry.
Optimal working dilutions must be determined by the end user.
Target description
90 kDa
Linkage
Replaces: AB5471
Physical form
Affinity Purified immunoglobulin. Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
ImmunoAffinity Purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Embryonic brain tissue, neural growth cones
Embryonic brain tissue, neural growth cones
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Endogenous axon guiding chemorepulsant semaphorin-3F inhibits the growth and metastasis of colorectal carcinoma.
Wu, F; Zhou, Q; Yang, J; Duan, GJ; Ou, JJ; Zhang, R; Pan, F; Peng, QP; Tan, H; Ping et al.
Clinical cancer research : an official journal of the American Association for Cancer Research null
Expression of class 3 semaphorins and their receptors in human breast neoplasia.
Carolyn A Staton,Lucy A Shaw,Manoj Valluru,Leslie Hoh,Ivan Koay,Simon S Cross et al.
Histopathology null
Identification of genes differentially expressed in rat alveolar type I cells
Dahlin, Katherine, et al.
American Journal of Respiratory Cell and Molecular Biology, 31, 309-316 (2004)
Alexander Fassold et al.
Arthritis and rheumatism, 60(10), 2892-2901 (2009-10-01)
In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of
Benoit Favier et al.
Blood, 108(4), 1243-1250 (2006-04-20)
Neuropilin 2 (NRP2) is a receptor for the vascular endothelial growth factor (VEGF) and the semaphorin (SEMA) families, 2 unrelated ligand families involved in angiogenesis and neuronal guidance. NRP2 specifically binds VEGF-A and VEGF-C, although the biological relevance of these
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