525200-M

Phospholipase D, Streptomyces chromofuscus

• Synonym(s): Phospholipase D, Streptomyces chromofuscus, PLD
• CAS Number: 9001-87-0
• Enzyme Commission number: 3.1.4.4 (BRENDA, IUBMB)
• MDL number: MFCD00131897
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• form: lyophilized solid
• Quality Level: 100
• specific activity: ≥50 units/mg dry wt.
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• color: brownish
• solubility: 10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON^™ X-100: 1 mg/mL
• foreign activity: Aatalase, glucose oxidase, none detected
• storage temp.: −20°C

Description

General description

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline; coupled with choline oxidase, it can be used for the determination of phospholipids in serum.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.

Other Notes

Kurz, T., et al. 1993. Circ. Res. 72, 701.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3