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Key Documents

524742

Sigma-Aldrich

Phosphodiesterase 3A, Active, Human, Recombinant, S. frugiperda

Synonym(s):

cAMP-specific phosphodiesterase 3A, PDE3A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.54

recombinant

expressed in insect cells (S. frugiperda)

Quality Level

100

form

liquid

specific activity

≥0.5 U/μL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Other Notes

Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XVII (Restriction List)

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

Certificates of Analysis (COA)

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