33-036

PI 3-Kinase HTRF^® Assay; 10,000 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 10,000 wells.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 100
• species reactivity: mouse, human
• manufacturer/tradename: HTRF^®; Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_006218.2
• UniProt accession no.: P42336
• detection method: fluorometric (HTRF)
• shipped in: dry ice
• Gene Information: human ... PIK3CA(5290)

Description

General description

Phosphoinositide (PI) 3-kinase (PI3K) is a heterodimeric lipid kinase containing regulatory and catalytic subunits. It catalyzes the phosphorylation at the 3-position hydroxyl group of the inositol ring of phosphatidylinositol (PtdIns). PI3K is implicated in various cellular functions such as cell growth, proliferation, motility, differentiation, survival, apoptosis, cytoskeletal rearrangement, and intracellular trafficking. It also plays a vital role in glucose uptake triggered by insulin.

Application

PI 3-Kinase HTRF^® Assay; 10,000 wells has been used for measuring the enzymatic activity of the class I phosphoinositide (PI) 3-kinase isoforms by a time-resolved fluorescence resonance energy transfer (TR-FRET) assay.

Biochem/physiol Actions

Target Sub-Family: PI3K/PI4K

Packaging

Kit capacity: 10,000 wells

Components

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

Storage and Stability

12 months at -70°C

Legal Information

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.