1.00289

L-Glutamine

for biochemistry

• Synonym(s): L-Glutamic acid-5-amide, Gln, (S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide
• Linear Formula: H₂NCOCH₂CH₂CH(NH₂)CO₂H
• CAS Number: 56-85-9
• Molecular Weight: 146.14
• Beilstein: 1723797
• MDL number: MFCD00008044
• UNSPSC Code: 12352209
• EC Index Number: 200-292-1

Properties

• Quality Level: 100
• form: solid
• potency: 7500 mg/kg LD₅₀, oral (Rat)
• pH: 4.0-6.0 (20 °C, 25 g/L in H₂O)
• mp: 185 °C (dec.) (lit.)
• solubility: 100 g/L
• density: 1.47 g/cm³ at 20 °C
• bulk density: 640 kg/m³
• storage temp.: 2-30°C
• SMILES string: N[C@@H](CCC(N)=O)C(O)=O
• InChI: 1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
• InChI key: ZDXPYRJPNDTMRX-VKHMYHEASA-N

Description

Application

• Low-protein diet supplemented with 1% L-glutamine improves growth performance, serum biochemistry, redox status, plasma amino acids, and alters fecal microbiota in weaned piglets.: This study demonstrates how L-glutamine supplementation in a low-protein diet can significantly enhance growth metrics and overall health in weaned piglets (Li et al., 2024).


• Functionalization of β-cyclodextrin metal-organic frameworks with gelatin and glutamine for drug delivery of curcumin to cancerous cells.: This article covers the development of a novel drug delivery system using glutamine-modified frameworks for targeted cancer therapy (Sadeh et al., 2024).

Biochem/physiol Actions

L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Analysis Note

Assay (perchloric acid titration, calculated on dried substance): 99.0 - 101.0 %
Identity (IR-spectrum): passes test
Appearance: white to almost white, fine powder
Appearance of solution (25 g/l; CO₂-free water): clear and colorless
Spec. rotation (α 20/D, 100 g/l, hydrochloric acid 2 mol/l, calc. on dried substance): +31,5 to +33,0
Chloride (Cl): ≤ 200 ppm
Sulfate (SO₄): ≤ 200 ppm
Heavy metals (as Pb): ≤ 10 ppm
As (Arsenic): ≤ 5 ppm
Ca (Calcium): ≤ 10 ppm
Co (Cobalt): ≤ 5 ppm
Fe (Iron): ≤ 5 ppm
K (Potassium): ≤ 5 ppm
Mg (Magnesium): ≤ 5 ppm
Na (Sodium): ≤ 50 ppm
Zn (Zinc): ≤ 5 ppm
Ninhydrin-positive substances (LC)(any ninhydrin-positive impurity): ≤ 0.3 %
Ninhydrin-positive substances (LC) (ammonium (570 nm)): ≤ 0.1 %
Ninhydrin-positive substances (LC)(total impurities): ≤ 0.5 %
Loss on drying (105 °C; 3 h): ≤ 0.3 %

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable