- A Chemical-Induced, Seed-Soaking Activation Procedure for Regulated Gene Expression in Rice.
A Chemical-Induced, Seed-Soaking Activation Procedure for Regulated Gene Expression in Rice.
Frontiers in plant science (2017-09-06)
Zaijie Chen, Qianqian Cheng, Chanquan Hu, Xinrui Guo, Ziqiang Chen, Yan Lin, Taijiao Hu, Maria Bellizzi, Guodong Lu, Guo-Liang Wang, Zonghua Wang, Songbiao Chen, Feng Wang
PMID28871269
ABSTRACT
Inducible gene expression has emerged as a powerful tool for plant functional genomics. The estrogen receptor-based, chemical-inducible system XVE has been used in many plant species, but the limited systemic movement of inducer β-estradiol in transgenic rice plants has prohibited a wide use of the XVE system in this important food crop. Here, we constructed an improved chemical-regulated, site-specific recombination system by employing the XVE transactivator in combination with a Cre/
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