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Photochemistry and mechanism of designed pyrenyl probe towards promoted cleavage of proteins.

Journal of photochemistry and photobiology. B, Biology (2017-05-30)
Sudarat Yenjai, Mayuso Kuno, Siritron Samosorn, Teerayuth Liwporncharoenvong, Apinya Buranaprapuk
ABSTRACT

A new photochemical reagent, succinic acid-1(1-pyrene)methylamide (PMA-SUC), was developed to recognize the specific binding sites on model proteins, egg-white lysozyme and avidin. The interaction of the photochemical reagent with the proteins was studied by UV-Vis, fluorescence spectroscopic methods and docking description. PMA-SUC was found to bind to lysozyme and avidin with binding constants (K

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Sigma-Aldrich
1-Pyrenemethylamine hydrochloride, 95%