A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

A colloidal gold nanoparticle-based immunochromatographic test strip for rapid and convenient detection of Staphylococcus aureus.

Journal of nanoscience and nanotechnology (2014-04-25)

Kaili Niu, Xiaoping Zheng, Chusen Huang, Kuan Xul, Yuan Zhi, Hebai Shen, Nengqin Jia

ABSTRACT

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

265810

Sigma-Aldrich

Gold, foil, thickness 0.1 mm, 99.99% trace metals basis

265772

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Gold, powder, <45 μm, 99.99% trace metals basis

326585

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Gold, powder, <10 μm, ≥99.9% trace metals basis

636347

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Gold, nanopowder, <100 nm particle size, 99.9% trace metals basis

326534

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Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis

373168

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Gold, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis

268461

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Gold, foil, thickness 0.025 mm, 99.99% trace metals basis

326496

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Gold, foil, thickness 0.127 mm, 99.99% trace metals basis

326542

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Gold, beads, 1-6 mm, 99.999% trace metals basis

643203

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Gold coated glass slide, layer thickness 100 Å, 99.999% (Au)

GF96742479

Gold, rod, 50mm, diameter 3.0mm, as drawn, 99.95%

310980

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Gold, wire, diam. 0.5 mm, 99.99% trace metals basis

349305

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GF08843867

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643246

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Gold coated glass slide, layer thickness 1000 Å, 99.999% (Au)

265829

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Gold, foil, thickness 0.5 mm, 99.99% trace metals basis

265837

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Gold, rod, diam. 3.0 mm, 99.99% trace metals basis

255734

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Gold, powder, <850 μm, ≥99.99% trace metals basis

373184

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Gold, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis

643254

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Gold coated glass cover slip, 99.999% (Au), L × W × thickness 22 mm × 22 mm × 130-170 μm, square, layer thickness 100 Å

349283

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Gold, wire, diam. 0.1 mm, 99.99% trace metals basis

265799

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Gold, wire, diam. 1.0 mm, 99.99% trace metals basis

GF98812097

Gold, rod, 6mm, diameter 6.0mm, as drawn, 99.95%

349275

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GF77657574

Gold, sphere, 20pcs, diameter 0.5mm, 99.99%

349313

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Gold, wire, diam. 0.5 mm, 99.999% trace metals basis

643289

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Gold coated glass cover slip, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å

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Gold, tube, 50mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%

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349240

Sigma-Aldrich

Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis