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Methyl green and its analogues bind selectively to AT-rich regions of native DNA.

European journal of histochemistry : EJH (1992-01-01)
K R Fox, S L Higson, J E Scott
ABSTRACT

Methyl green has long been used as a DNA stain in histochemistry. The sequence selective binding of the cationic triphenylmethane dyes methyl green, crystal violet and Malachite green to DNA was investigated by DNAase 1 and micrococcal nuclease footprinting. At low concentrations the ligands showed similar footprinting patterns which centred around AT-rich regions with a mild preference for hompolymeric A and T. At higher concentrations the dyes bound to almost all available DNA sites. Models, with and without intercalation are discussed to account for the specific binding.

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Sigma-Aldrich
Methyl Green, zinc chloride salt, for microscopy (Bact., Bot., Hist.)