[Protective effects of catalpol against H2O2-induced oxidative damage in astrocytes].

To study the protective effects of catalpol against H2O2-induced oxidative damage in astrocytes. Astrocytes were pretreated with 1, 10, 100 micromol x L(-1) catalpol and then exposed to H2O2. Effects of catalpol on cell morphous were observed by inverted microscope. Cell viability were detected by MTT assay. LDH content in the cell culture fluid, and intracellular T-SOD, GSH-Px activity and MDA content were assessed by colorimetric method. The results showed that catalpol evidently ameliorated cell morphous, improved cell viability, and maintained integrality of cell membrane. Furthermore, catalpol could significantly inhibit the decrease of intracellullar T-SOD and GSH-Px activity, and the increase of MDA content. Catalpol have protective effects against H2O2-induced oxidative damage in astrocytes.