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Merck

Optimal enzymatic hydrolysis of urinary benzodiazepine conjugates.

Journal of analytical toxicology (1994-11-01)
R Meatherall
ABSTRACT

Conditions for the enzymatic hydrolysis of benzodiazepine conjugates were systematically examined. Optimal recovery of the free drugs occurs when 1 mL of urine buffered to pH 4.5 is incubated with 5000 U of Helix pomatia beta-glucuronidase at 56 degrees C for 2 h. Urine specimens containing conjugates of temazepam, oxazepam, lorazepam, alpha-hydroxyalprazolam, 2-hydroxyethylflurazepam, and N-desalkyl-3-hydroxyflurazepam as targeted benzodiazepines were used. The freed drugs were quantitated by gas chromatography-mass spectrometry.

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Sigma-Aldrich
β-Glucuronidasi, Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)