CKT2MAG-97K

MILLIPLEX^® Canine Kidney Toxicity Panel 2 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: Albumin, β-2-microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)

• Codice UNSPSC: 12161503
• eCl@ss: 32161000

Proprietà

• Livello qualitativo: 200
• Reattività contro le specie: canine
• Produttore/marchio commerciale: Milliplex^®
• assay range: accuracy: 96-102%; sensitivity: 0.016-0.026 ng/mL; (All other analytes; MinDC +2SD); sensitivity: 12.48 ng/mL; (Albumin; MinDC +2SD); standard curve range: 0.01-50 ng/mL; (RBP4); standard curve range: 0.02-100 ng/mL; (B2M); standard curve range: 0.02-100 ng/mL; (TFF-3); standard curve range: 12.21-50,000 ng/mL; (Albumin)
• tecniche: multiplexing: suitable
• Metodo di rivelazione: fluorometric (Luminex xMAP)
• Condizioni di spedizione: wet ice

Descrizione

Descrizione generale

As one of the leading causes of drug candidate attrition, toxicity research is receiving more attention, especially in the search of sensitive, organ-specific toxicity biomarkers. In pharmaceutical and chemical industries, renal function is routinely assessed during toxicity evaluations. The importance of the kidney as a central detoxification organ leads to a high exposure of renal tissue to drugs, reactive metabolites, and environmental compounds. Traditional markers for assessing renal toxicity, such as blood urea nitrogen (BUN) and serum creatinine, are insensitive and non-specific. For improved, non-invasive detection of acute nephrotoxicity, a panel of novel urinary kidney biomarkers has been identified and validated for preclinical studies in relevant animal species.

MILLIPLEX^® Canine Kidney Toxicity Panel 2 is a 4-plex kit to be used for the simultaneous quantification of any combination of the following analytes in canine urine samples: Albumin*, β-2-microglobulin (B2M)*, Trefoil Factor 3 (TFF-3)*, Retinol Binding Protein 4 (RBP4)*. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
*Urinary albumin, B2M, TFF-3 and RBP4 are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Toxicity

Specificità

Cross Reactivty
Approximately 5% cross-reactivity may be observed between B2M beads and TFF-3 protein. No other significant cross-reactivity was observed for other analytes in this panel.

Applicazioni

• Analytes: Albumin, β-2-Microglobulin (B2M), Trefoil Factor 3 (TFF-3), Retinol Binding Protein 4 (RBP4)
• Recommended Sample Type: Canine urine
• Recommended Sample Dilution: 25 μL of 1:500 diluted canine urine for normal subjects; optimal dilutions for disease subjects should be determined
• Assay Run Time: Primary incubation of 2 hours, followed by a secondary incubation of 1 hour, both at room temperature (20-25°C)
• Research Category: Toxicology

Caratteristiche e vantaggi

Design your multiplex kit by choosing available analytes within this panel.

Componenti

Canine Kidney Toxicity Panel 2 Standard, 1 vial, lyophilized

Canine Kidney Toxicity Panel 2 Control 1, 1 vial, lyophilized

Canine Kidney Toxicity Panel 2 Control 2, 1 vial, lyophilized

Assay Buffer, 2 bottles, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Canine Kidney Toxicity Panel 2 Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Altre note

Please contact Technical Service for linearity of dilution.

Sensitivity: See kit protocol for individual analyte sensitivities.

Note legali

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS06,GHS08,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H302 + H332 - H311 - H317 - H318 - H373 - H411
• Consigli di prudenza: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Classi di pericolo: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Organi bersaglio: Respiratory Tract
• Codice della classe di stoccaggio: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 3