1.10675
GranuCult® GIOLITTI-CANTONI broth (Base)
pkg of 500 g, ISO 6888, for Staphylococcus spp.
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
41106213
NACRES:
NA.74
Prodotti consigliati
agenzia
ISO 6888
Livello qualitativo
Forma fisica
medium granules (dehydrated (DCM))
Confezionamento
pkg of 500 g
Produttore/marchio commerciale
GranuCult®
Condizioni di stoccaggio
protect from light (both UV and Sunlight)
tecniche
microbiological culture: suitable
pH
6.8-7.0 (20 °C, 54 g/L in H2O, after autoclaving)
Solubilità
54 g/L
Densità bulk
500 kg/m3
applicazioni
food and beverages
microbiology
Temperatura di conservazione
15-25°C
Compatibilità
Staphylococcus spp. (spp.)
Descrizione generale
GranuCult® GIOLITTI-CANTONI broth (Base) promotes the growth of staphylococci due to the presence of pyruvate, glycine, Tween® 80, and a high concentration of mannitol. This medium stimulates the growth of injured staphylococci and aids detection of the organism when present in small numbers only. Lithium chloride inhibits the Gram-negative contaminants, while potassium tellurite and glycine inhibit the Gram-positive contaminants. Furthermore, anaerobiosis suppresses the micrococci to a certain degree. Black coloration or black precipitate of the culture medium, due to reduction of tellurite to metallic tellurium, indicates the growth of staphylococci.
Applicazioni
GranuCult® GIOLITTI-CANTONI broth (Base) is suitable for the enumeration and detection of coagulase-positive staphylococci (Staphylococcus aureus and other species) from food and animal feeding stuff as well as from environmental samples in food production and food handling.
Risultati analitici
Appearance (clearness): clear
Appearance (color): yellowish-brown
pH-value (25 °C): 6.7 - 7.1
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of S.aureus and E.coli.
Additionaly monoculture of E.coli.
Confirmation on Baird-Parker-Agar resp. Tryptic soy agar
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 10000
Growth on Baird-Parker-Agar(Staphylococcus aureus ATCC 6538 (WDCM 00032)): >10 colonies
Growth on Baird-Parker-Agar(Staphylococcus aureus ATCC 25923 (WDCM 00034)): >10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Incubation
24 ± 2 hours at 37 ± 1 °C aerobic.
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic.
Confirmation on Baird-Parker-Agar: 48 ± 2 hours at 37 ± 1 °C aerobic.
Confirmation on Tryptiy Soy-Agar: 24 ± 2 hours at 37 ± 1 °C aerobic.
Appearance (color): yellowish-brown
pH-value (25 °C): 6.7 - 7.1
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of S.aureus and E.coli.
Additionaly monoculture of E.coli.
Confirmation on Baird-Parker-Agar resp. Tryptic soy agar
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≤ 100
Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 10000
Growth on Baird-Parker-Agar(Staphylococcus aureus ATCC 6538 (WDCM 00032)): >10 colonies
Growth on Baird-Parker-Agar(Staphylococcus aureus ATCC 25923 (WDCM 00034)): >10 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Colonies (Staphylococcus aureus ATCC 25923 (WDCM 00034)): Black or grey colonies with clear halo
Colonies (Staphylococcus aureus ATCC 6538 (WDCM 00032)): Black or grey colonies with clear halo
Incubation
24 ± 2 hours at 37 ± 1 °C aerobic.
E.coli 48 ± 2 hours at 37 ± 1 °C aerobic.
Confirmation on Baird-Parker-Agar: 48 ± 2 hours at 37 ± 1 °C aerobic.
Confirmation on Tryptiy Soy-Agar: 24 ± 2 hours at 37 ± 1 °C aerobic.
Nota a piè di pagina
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Note legali
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Skin Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.