HPLC Analysis of Water Soluble Vitamins on Ascentis® RP-Amide
CONDITIONS
column
Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase
25 mM potassium phosphate monobasic, pH 3.5
flow rate
1.0 mL/min
column temp.
30 °C
detector
UV, 230 nm
injection
10 μL
Description
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany