ZRB2055

Anti-Fibrillarin/FBL Antibody, clone 1G19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2′-O-methyltransferase fibrillarin, rRNA 2′-O-methyltransferase fibrillarin
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1G19, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Produit purifié par: using Protein A
• Espèces réactives: mouse, rat, human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: N-terminal half
• Numéro d'accès Protein ID: NP_001427
• Numéro d'accès UniProt: P22087
• Autre catégorie plus écologique: , Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: human ... FBL(2091)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1G19 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Fibrillarin/FBL. It targets an epitope within 15 amino acids from the N-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Fibrillarin.

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Fibrillarin/FBL in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Fibrillarin/FBL in Neuro2a and PC12 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound Fibrillarin/FBL peptide with a KD of 2.0 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrillarin/FBL in Human small intestine tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Fibrillarin/FBL in HEK293 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

rRNA 2′-O-methyltransferase fibrillarin (UniProt: P22087; also known as 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2′-O-methyltransferase fibrillarin) is encoded by the FBL (also known as FIB1, FLRN) gene (Gene ID: 2091) in human. Fibrillarin is a highly conserved nucleolar protein that is a component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that also contain SNU13, NOP5 and NOP56, and a guide RNA. It is one of the most abundant proteins present in nucleoli. It serves as a S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. It is involved in several methylation sites in rRNA that are essential for proper ribosome function. Its site specificity is provided by a guide RNA that base pairs with the substrate. Fibrillarin involved methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. It is also reported to ACT^® as a protein methyltransferase by mediating methylation of Gln-105 of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus. Its acetylation by CREBBP/CBP prevents methylation of Gln-105 of histone H2A, without affecting rRNA methylation. Its deacetylation by SIRT7 restores methylation of H2AQ104me. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Iyer-Bierhoff, A., et al. (2018). Cell Rep. 25(11); 2946-2954; Tessarz, P., et al. (2014). Nature. 505(7484); 564-568).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ACT is a registered trademark of Merck KGaA, Darmstadt, Germany

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable