ZMS1166

Anti-Elastin Antibody, clone 10B8 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Tropoelastin
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 10B8, recombinant monoclonal
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 63.7 kDa; observed mol wt ~55 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): bovine, mouse, chicken
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NM_000501.2
• Numéro d'accès UniProt: P07916
• Autre catégorie plus écologique: Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Informations sur le gène: chicken ... ELN(396441)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 10B8 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects elastin.

Immunogène

Full-length, purified elastin from aorta of 17-day old lathyric chick embryo.

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse heart tissue membranes..

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Elastin in Moue heart tissue membranes.

Tested Applications

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Elastin in Human lung tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 from a representative lot detected Elastin in Human skin and Human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Elastin (UniProt: P07916; also known as Tropoelastin) is encoded by the ELN gene (Gene ID: 396441) in chicken. Elastin, an extracellular matrix (ECM) protein, is responsible for the extensibility and elastic recoil of many vertebrate tissues, such as large arteries, heart valves, pulmonary tissues, skin, and certain ligaments, and cartilages. It is about 1000-fold more flexible than collagens. Elastin is formed through the cross-linking of its soluble precursor tropoelastin, which is derived from fibroblasts, smooth muscle cells, chondrocytes, or endothelial cells. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.  Tropoelastin contains alternating hydrophobic and cross-linking domains, and both contribute to the proper supramolecular assembly and the mechanical properties of the polymeric elastin network. The cross-linking domains provide cohesiveness and durability, and the hydrophobic domains confer the propensities for self-assembly and recoil properties. In a stable state, the basal levels of elastin expression and breakdown are reported to be low in adult tissues and in cells. Mutations in the elastin gene may lead to diseases such as Williams–Beuren syndrome, cutis laxa, and supravalvular aortic stenosis (SVAS). This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, K., et al. (2021). Front. Cell Dev. Biol. 9; 596702; Rauscher, S., and Pomes, R. (2017). Elife. 6; e26526).

Forme physique

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable