Y2377

2x YT medium

Powder microbial growth medium

• Synonyme(s) : 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
• Code UNSPSC : 41106225
• Nomenclature NACRES : NA.85

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Stérilité: non-sterile
• Forme: powder
• Technique(s): microbiological culture: suitable
• pH: 6.8-7.2(3.1% solution)
• Application(s): food and beverages; microbiology
• Température de stockage: room temp
• Adéquation: nonselective for Escherichia coli; nonselective for coliforms

Description

Description générale

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Caractéristiques et avantages

2xYT powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Composants

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Notes préparatoires

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Reconstitution

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)