Accéder au contenu
Merck
Toutes les photos(2)

Documents

W1754

Sigma-Aldrich

Eau

PCR Reagent, suitable for PCR

Synonyme(s) :

H2O

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Formule linéaire :
H2O
Numéro CAS:
7732-18-5
Poids moléculaire :
18.02
Numéro Beilstein :
2050024
Numéro CE :
231-791-2
Numéro MDL:
MFCD00011332
Code UNSPSC :
12191602
ID de substance PubChem :
24900779
Nomenclature NACRES :
NA.25

product name

Eau, PCR Reagent

Qualité

PCR Reagent

Densité de vapeur

<1 (vs air)

Pression de vapeur

3 mmHg

Stérilité

sterile-filtered

Forme

liquid

Conditionnement

vial of 1.5 mL

Technique(s)

PCR: suitable

Indice de réfraction

n20/D 1.34 (lit.)

pH

5-7

Point d'ébullition

100 °C (lit.)

Pf

0 °C (lit.)

Densité

1.000 g/mL at 3.98 °C (lit.)

Activité étrangère

DNase, none detected
RNase, none detected

Chaîne SMILES 

O

InChI

1S/H2O/h1H2

Clé InChI

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Description générale

PCR grade water is sterile-filtered. It is free from exonucleases (DNAse, RNAse) and endonuclease (NICKase) and is also free from nucleic acid contamination.

Application

Water has been used:

  • as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
  • as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
  • as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
  • Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)

Adéquation

Suitable for polymerase chain reaction (PCR)

Autres remarques

Easily compare specifications for Water products with the Water specification table.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 8

1 of 8

Eau for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture

Sigma-Aldrich

W1503

Eau

Eau OmniPur&#174; Grade, DEPC Treated, Autoclaved, Nuclease-Free

Sigma-Aldrich

9601-OP

Eau

Eau Sterile-filtered water treated with diethyl pyrocarbonate (DEPC).

Sigma-Aldrich

693520

Eau

Eau ACS reagent

Sigma-Aldrich

320072

Eau

Eau Deionized

Sigma-Aldrich

38796

Eau

Eau ACS reagent, for ultratrace analysis

Supelco

14211

Eau

Eau for inorganic trace analysis Suprapur&#174;

Supelco

1.00473

Eau

Eau suitable for HPLC

Sigma-Aldrich

270733

Eau

J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed.
Francine Marciano-Cabral et al.
Applied and environmental microbiology, 69(10), 5864-5869 (2003-10-09)
The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Afficher tous les articles scientifiques apparentés

Articles

Examination of Efficacy, Specificity, and Efficiency of an shRNA Lentiviral Library

The introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Examination of Efficacy, Specificity and Efficiency of an shRNA Lentiviral Library

Introduction of small interfering RNAs (siRNAs) into cultured cells provides a fast and efficient means of knocking down gene expression and has allowed siRNAs to quickly become a ubiquitous tool in molecular biology.

Protocoles

Hot Start dNTP protocol to reduce non-specific amplification

Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.

Long and Accurate PCR Amplification of DNA (D8045)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Afficher tout

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique