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T6778

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–TRITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Goat Anti-Rabbit IgG (whole molecule)–Tetramethylrhodamine isothiocyanate

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About This Item

Numéro MDL:
MFCD00162790
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

goat

Niveau de qualité

200

Conjugué

TRITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Contient

≥15 mM sodium azide as preservative

Conditions de stockage

protect from light

Technique(s)

direct immunofluorescence: 1:400
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

Binds all rabbit Igs.
IgG consists of four isotypes IgG1, IgG2, IgG3 and IgG4. It contains four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds.

Application

Anti-Rabbit IgG (whole molecule)−TRITC antibody produced in goat has been used in immunocytochemistry.
Conjugation of TRITC to Rabbit IgG (whole molecule) antibody enables the use in immunofluorescent techniques.

Actions biochimiques/physiologiques

IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis. It might be associated with allergy. IgG mediates transplacental transport and participates in the secondary immune response. It has the longest serum half life.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Autres remarques

Antibody adsorbed with human IgG.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Notes préparatoires

Adsorbed to reduce background staining with human samples.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Goeh Jung et al.
The Journal of cell biology, 186(4), 555-570 (2009-08-19)
The contractile vacuole (CV) complex in Dictyostelium is a tubulovesicular osmoregulatory organelle that exhibits extensive motility along the actin-rich cortex, providing a useful model for investigating myosin-dependent membrane transport. Here, we show that the type V myosin myoJ localizes to
Kamin J Johnson et al.
Biology of reproduction, 66(4), 992-1000 (2002-03-22)
In the seminiferous epithelium, morphologically diverse junctions mediate inter-Sertoli and Sertoli-germ cell adhesive contact and likely transmit signals between contacting cells. Defining the molecular composition of testicular cell-cell junctions is an important step in determining their function. Proteins belonging to
5-HT1B and 5-HT1D receptors in the human trigeminal ganglion: co-localization with calcitonin gene-related peptide, substance P and nitric oxide synthase
Hou M, et al.
Brain Research, 909(1-2), 112-120 (2001)
Daniel H Fine et al.
Infection and immunity, 73(4), 1947-1953 (2005-03-24)
Cells of the gram-negative periodontopathogen Actinobacillus actinomycetemcomitans express a surface-exposed, outer membrane autotransporter protein, designated Aae, which has been implicated in epithelial cell binding. We constructed a mutant strain of A. actinomycetemcomitans that contained a transposon insertion in the Aae
Martin Hasselblatt et al.
Cancer research, 69(6), 2219-2223 (2009-03-12)
The pathogenesis of choroid plexus papillomas, intraventricular papillary neoplasms most often occurring sporadically in children and young adults, remains poorly understood. To identify pathways operative in the development of choroid plexus papillomas, gene expression profiles obtained from laser-microdissected human choroid
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