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Key Documents

T2402

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)−TRITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Rabbit Anti-Mouse IgG (whole molecule)−Tetramethylrhodamine isothiocyanate

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Niveau de qualité

Conjugué

TRITC conjugate

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

direct immunofluorescence: 1:64
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.

Spécificité

Anti-Mouse IgG (whole molecule)-TRITC antibody is specific for mouse IgG.

Immunogène

Normal mouse serum

Application

Anti-Mouse IgG (whole molecule)-TRITC antibody is suitable for use in direct immunofluorescence (1:64), immunohistochemistry (1:200 dilution in formalin-fixed, paraffin-embedded sections), Ouchterlony double diffusion and electrophoresis techniques.

Actions biochimiques/physiologiques

Digestion of IgG by papain results in generation of fragment antigen binding (Fab) comprising one complete L chain and a variable and CH1 region of H chain. Pepsin digestion of IgG results in fragment crystallizable (fc), comprises the H chain constant region.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice .

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Mikkel Wölck Misfeldt et al.
Investigative ophthalmology & visual science, 51(12), 6681-6687 (2010-08-07)
The retinal blood flow is regulated by the tone of resistance arterioles, which is influenced by purinergic compounds such as adenosine and adenosine 5'-triphosphate (ATP) released from the retinal tissue. However, it is unknown what cellular elements in the perivascular
Rajbir K Gill et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 14(22), 7481-7487 (2008-11-18)
We seek to establish a genetic test to identify lung cancer using cells obtained through computed tomography-guided fine needle aspiration (FNA). We selected regions of frequent copy number gains in chromosomes 1q32, 3q26, 5p15, and 8q24 in non-small cell lung
Jee Yoon Shin et al.
PloS one, 14(7), e0219800-e0219800 (2019-07-31)
It has been suggested that Jmjd6 plays an important role in gene regulation through its demethylation or hydroxylation activity on histone and transcription factors. In addition, Jmjd6 has been shown to regulate RNA splicing by interaction with splicing factors. In
Kathryn S Poland et al.
Genes, chromosomes & cancer, 46(9), 805-812 (2007-06-01)
Studying the molecular basis of familial renal cell carcinoma (RCC) has allowed identification of novel RCC genes involved in the pathogenesis of both inherited and sporadic RCC. We describe a constitutional balanced t(3;8)(p14;q24.1) translocation found in a brother and sister
Jessica E Poort et al.
Human molecular genetics, 25(17), 3768-3783 (2016-08-06)
Spinal bulbar muscular atrophy (SBMA) is a progressive, late onset neuromuscular disease causing motor dysfunction in men. While the morphology of the neuromuscular junction (NMJ) is typically affected by neuromuscular disease, whether NMJs in SBMA are similarly affected by disease

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