SRP6546

Procathepsin L human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : CTSL, CTSL1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥90% (SDS-PAGE)
• Forme: liquid
• Poids mol.: 39.4 kDa (18-333 aa + N-terminal poly-his tag.)
• Conditionnement: pkg of 1 mg; pkg of 100 μg
• Concentration: 1 mg/mL
• Numéro d'accès UniProt: P07711
• Conditions d'expédition: wet ice
• Température de stockage: −70°C
• Informations sur le gène: human ... CTSL1(1514)

Description

Description générale

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

Forme physique

1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable