SRP6481

CTLA4/CD152 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : CELIAC3, GRD4, GSE, ICOS, IDDM12
• Code UNSPSC : 12352200

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Étiquette/Marqueur: 6-His tagged (C-terminus)
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: calculated mol wt 14.3 kDa; observed mol wt 25 kDa by SDS-PAGE (DTT-reduced. Ala 37 is the predicted N-terminal.)
• Conditionnement: pkg of 20 and 100 μg
• Impuretés: <1 EU/μg endotoxin (LAL test)
• Numéro d'accès UniProt: P16410
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CTLA4(1493)

Description

Description générale

CTLA4 (cytotoxic T-lymphocyte associated protein 4) or CD152 (cluster of differentiation) is a T cell receptor which belongs to the immunoglobulin (Ig) superfamily or CD28 family. It is expressed on the surface of Th (T helper) cells. This gene is localized to human chromosome 2q33. This protein is composed of a cytosolic domain, a transmembrane region, and a V domain of 116 amino acids. Alternative splicing results in membrane and soluble forms, where membrane forms work as homodimers and soluble isoforms as monomers.

Actions biochimiques/physiologiques

CTLA4 (cytotoxic T-lymphocyte associated protein 4) is responsible for inhibiting T-cell proliferation and facilitates T cell apoptosis. This protein is expressed post-antigen presentation, and thus, is involved in immune and auto-immune disorders. Therefore, it is also implicated in the pathogenesis of various T cell mediated autoimmune disorders. Studies in Baghdadi or Iraqi population show that polymorphism in this gene is linked with susceptibility to diabetes mellitus type I.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable