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Key Documents

SRP6267

Sigma-Aldrich

C-reactive/CRP human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonyme(s) :

C-reactive protein, CRP, PTX1

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in HEK 293 cells

Étiquette/Marqueur

6-His tagged (C-terminus)

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

calculated mol wt 24 kDa
observed mol wt 26 kDa (DTT-reduced. Phe17 is the predicted N-terminus.)

Conditionnement

pkg of 100 μg

Impuretés

<1 EU/μg endotoxin (LAL test)

Numéro d'accès UniProt

P02741

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... CRP(1401)

Description générale

C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat, respectively.

Actions biochimiques/physiologiques

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Function of C-reactive protein
Du Clos TW
Annals of Medicine, 32, 274-278 (2000)
Human C-reactive protein: expression, structure, and function.
Volanakis JE
Molecular Immunology, 38, 189-197 (2001)
C-reactive protein attenuates endothelial progenitor cell survival, differentiation, and function: further evidence of a mechanistic link between C-reactive protein and cardiovascular disease.
Verma S
Circulation, 109, 2058-2067 (2004)

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