SRP6255

VEGF-B human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : VEGFB, VEGFL, VRF
• Code UNSPSC : 12352200

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Étiquette/Marqueur: 6-His tagged (C-terminus)
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized powder
• Poids mol.: calculated mol wt 20.2 kDa; observed mol wt 15-18 kDa (DTT-reduced. Protein migrates due to glycosylation. Arg 22 is the predicted N-terminus.); observed mol wt 33-35 kDa
• Conditionnement: pkg of 10 μg
• Impuretés: <1 EU/μg endotoxin (LAL test)
• Numéro d'accès UniProt: P49765
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... VEGF-B(7423)

Description

Description générale

VEGFB (vascular endothelial growth factor B) is encoded by the gene mapped to human chromosome 11q13. The encoded protein is one of the seven members of the VEGF family, which also includes VEGF-A, VEGF-C, VEGF-D, VEGF-E, VEGF-F, and PIGF (placental growth factor). All these members contain a common VEGF homology domain. This domain forms the core region and contains a cystine knot motif composed of eight invariant cysteine residues. VEGFB, discovered in 1995, is highly expressed in adult myocardium, skeletal muscle, and pancreas. This gene contains seven exons and alternative splicing results in the synthesis of two isoforms, VEGF-B167 and VEGF-B186, with molecular weight of 21kDa and 32kDa, respectively. These isoforms show differential level of expression, with VEGF-B167 being the predominantly expressed isoform.

Actions biochimiques/physiologiques

VEGFB (vascular endothelial growth factor B) acts on the receptors VEGFR-1 (VEGF receptor) and NRP-1 (neuropilin). This protein induces coronary vessel growth and cardiac hypertrophy, which might confer protection to heart against ischemic damage and heart failure. It might have a role in metabolic functions as it shows high expression levels in tissues with highly active energy metabolism. VEGFB is also thought to be involved in neuroprotection. This protein is involved in fetal angiogenesis, functions as a mitogen for endothelial cells (ECs), survival factor for multiple types of cells such as neurons, vascular cells and myocytes.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable