SRP5086

SLK, active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonyme(s) : KIAA0204, LOSK, MGC133067, STK2, bA16H23.1, se20-9
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf9 cells
• Gamme de produits: PRECISIO^® Kinase
• Essai: ≥70% (SDS-PAGE)
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 3.9-5.3 nmol/min·mg
• Poids mol.: ~180 kDa
• Numéro d'accès NCBI: AB002804
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... SLK(9748)

Description

Description générale

SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex. Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity. CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates.

Forme physique

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Notes préparatoires

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Informations légales

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1