SRP4251

MIP-2 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonyme(s) : CXCL2, Gro-β, Growth- regulated protein β, MIP2-α, Macrophage inflammatory protein 2-α, chemokine (C-X-C motif) ligand 2
• Numéro MDL: MFCD01094259
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: mouse
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: ~7.8 kDa
• Conditionnement: pkg of 20 μg
• Impuretés: endotoxin, tested
• Numéro d'accès NCBI: P10889
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: mouse ... Cxcl2(20310)

Description

Description générale

MIP-2 (microphage inflammatory protein-2), or Cxcl2 ((C-X-C motif) ligand 2), was initially recognized as a 6kDa protein released by an LPS (lipopolysaccharide)-stimulated mouse macrophage cell line. It belongs to the chemokine family which contains 6-14kDa, heparin-binding cytokines. It is a member of the a (CXC) chemokine family, where any amino acid separates both conserved cysteines.
Mouse MIP-2 is a 7.8kDa protein containing 73 amino acid residues including the ‘RLR′ motif common to the CXC chemokine family that bind to CXCR1 or CXCR2.

Actions biochimiques/physiologiques

MIP-2 (microphage inflammatory protein-2), or Cxcl2 ((C-X-C motif) ligand 2), is a functional homolog of human IL-8 (interleukin) in mice, and like human IL-8 it induces a potent neutrophil chemotactic activity. It might be critical in the recruitment and influx of immune cells in inflammation and infection. Murine encephalomyelitis virus of Theiler (TMEV) induces the expression of MIP-2 in genetically susceptible but not in resistant mouse strains. In mice, in acute and chronic liver injury, TLR2 (Toll-like receptor)/S100A9/MIP-2 signaling pathways is crucial for neutrophil recruitment, and this might have therapeutic implications in selectively manipulating neutrophils in liver disease while circumventing normal wound healing and regenerative responses.

Forme physique

Sterile filtered and then lyophilized without additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1 mg/mL. The solution can be diluted into other aqueous buffers.