SRP3239
GDNF from rat
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
ATF-1, Glial-Derived Neurotrophic Factor
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
rat
Produit recombinant
expressed in E. coli
Pureté
≥98% (HPLC)
≥98% (SDS-PAGE)
Forme
lyophilized
Poids mol.
15 kDa
Conditionnement
pkg of 10 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Couleur
white to off-white
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
rat ... GDNF(25453)
Description générale
GDNF (glial cell derived neurotrophic factor) is a member of the transforming growth factor-β (TGF) superfamily. It is a potent neurotrophic factor which functions as a ligand for the multicomponent receptor complex containing GFRα1 (GDNF family receptor α1) and Ret receptor tyrosine kinase or neuronal cell adhesion molecule (NCAM). Rat GDNF is a homodimer linked by a disulfide bridge, and contains two glycosylated polypeptide chains that are released from the 211-residue-long precursor following a proteolytic cleavage.
The functional rat GDNF ligand is a disulfide-linked homodimer, of two 15.0kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
The functional rat GDNF ligand is a disulfide-linked homodimer, of two 15.0kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Actions biochimiques/physiologiques
GDNF (glial cell derived neurotrophic factor) enhances motoneuron survival, and facilitates the survival of midbrain dopaminergic neurons which are damaged in degenerative diseases, such as Parkinson′s. In vivo and in vitro, the interaction of this protein with GDNF family receptor α1 and its co-receptor Ret tyrosine kinase, results in elevated neurite extension and survival of motor and sensory neurons. In rats with neuropathic pain, the injection of this protein in the locus coeruleus (LC) produces prolonged analgesia by increasing descending noradrenergic inhibition.
Séquence
MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI
Forme physique
Lyophilized from 10 mM Sodium Citrate.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
Finely Tuned Temporal and Spatial Delivery of GDNF Promotes Enhanced Nerve Regeneration in a Long Nerve Defect Model.
Marquardt LM et al
Tissue Engineering: Part A, 21(23-24), 2852-2864 (2015)
Glial cell line-derived neurotrophic factor-mediated enhancement of noradrenergic descending inhibition in the locus coeruleus exerts prolonged analgesia in neuropathic pain.
Kimura M et al
British Journal of Pharmacology, 172(10), 2469-2478 (2015)
Peripheral expression and biological activities of GDNF, a new neurotrophic factor for avian and mammalian peripheral neurons.
Trupp M et al
The Journal of Cell Biology, 130(1), 137-148 (1995)
M Trupp et al.
Nature, 381(6585), 785-789 (1996-06-27)
Glial-cell-line-derived neutrophic factor (GDNF) promotes the survival and phenotype of central dopaminergic noradrenergic and motor neurons, as well as various subpopulations of peripheral sensory and sympathetic neurons. GDNF is structurally related to members of the transforming growth factor (TGF)-beta superfamily
Weidong Xiao et al.
Molecular neurobiology, 50(2), 274-289 (2014-06-01)
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