SRP3185
VEGF-D human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
Synonyme(s) :
FIGF, Vascular Endothelial Growth Factor-D
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in HEK 293 cells
Pureté
≥95% (HPLC)
≥95% (SDS-PAGE)
Forme
lyophilized
Poids mol.
20.0-22.0 kDa
Conditionnement
pkg of 10 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Couleur
white
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
human ... FIGF(2277)
Description générale
VEGFD (vascular endothelial growth factor D), or c-fos-induced growth factor, is a member of the VEGF family of growth factors, and is composed of VEGF-homology domain, receptor binding domains, and pro-peptides in both terminals. It is secreted in the extracellular space in a full length form with both C- and N-terminal pro-peptides, which are cleaved off to produce the mature VEGFD form. It is an angiogenesis-related factor.
Recombinant human VEGF-D is a 26.2 kDa non-disulfide linked homodimeric protein consisting of two 117 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.
Recombinant human VEGF-D is a 26.2 kDa non-disulfide linked homodimeric protein consisting of two 117 amino acid polypeptide chains. Due to glycosylation the protein migrates as a 20.0-22.0 kDa band under non-reducing condition.
Application
VEGFD (vascular endothelial growth factor D) human has been used in for the treatment of buffalo luteal cell culture to determine the effect of VEGFD on the expression of lymphatic endothelial marker (LYVE1), thereby investigating the role of VEGFD in luteal lymphangeogenesis during different stages of estrous cycle.
Actions biochimiques/physiologiques
Mature VEGFD (vascular endothelial growth factor D) interacts with VEGFR-3, a tyrosine kinase receptor, with high affinity. This receptor predominantly resides on adult lymphatic endothelium and participates in lymphangiogenesis. VEGFD also interacts with Nrp-2 (neuropilin), a non-tyrosine kinase receptor, which functions as a lymphangiogenesis-associated factor. This factor promotes tumor lymphangiogenesis and hence, lymph node metastasis in various cancers, such as ovarian cancer. In patients with CRC (colorectal cancer), higher expression of VEGFD is linked with resistance to bevacizumab, and has potential as a biomarker for predicting the benefit of bevacizumab in CRC chemotherapy.
Séquence
FAATFYDIET LKVIDEEWQR TQCSPRETCV EVASELGKST NTFFKPPCVN VFRCGGCCNE ESLICMNTST SYISKQLFEI SVPLTSVPEL VPVKVANHTG CKCLPTAPRH PYSIIRR
Forme physique
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Daniela C García et al.
Zygote (Cambridge, England), 26(3), 242-249 (2018-06-09)
SummaryThe mammalian oviduct plays a pivotal role in the success of early reproductive events. The urokinase plasminogen activator system (uPAS) is present in the bovine oviduct and is involved in extracellular matrix remodelling through plasmin generation. This system can be
VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma.
Du LC et al
Reproductive Biology and Endocrinology, 12, 14-14 (2014)
Expression and localization of locally produced growth factors regulating lymphangiogenesis during different stages of the estrous cycle in corpus luteum of buffalo (Bubalus bubalis).
Ali I et al
Theriogenology, 81(3), 428-436 (2014)
Himadri Roy et al.
FEBS letters, 580(12), 2879-2887 (2006-04-25)
Angiogenesis is the process by which new blood vessels are formed from existing vessels. The vascular endothelial growth factors (VEGFs) are considered as key molecules in the process of angiogenesis. The VEGF family currently includes VEGF-A, -B, -C, -D, -E
Ellen C Breen
Journal of cellular biochemistry, 102(6), 1358-1367 (2007-11-06)
Vascular endothelial growth factor A (VEGF-A) belongs to a family of heparin binding growth factors that include VEGF-B, VEGF-C, VEGF-D, and placental-like growth factor (PLGF). First discovered for its ability to regulate vascular endothelial cell permeability, VEGF is a well-known
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