SRP3126

NP-1 Peptide

≥98% (HPLC), human recombinant, expressed in E. coli, suitable for cell culture

• Synonyme(s) : Cryptdin, HNP-1, Neutrophil Peptide-1, alpha Defensin-1
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• product name: NP-1 human, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: 3.4 kDa
• Conditionnement: pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: O14786
• Spectre d'activité de l'antibiotique: fungi; mycobacteria; viruses
• Mode d’action: cell membrane | interferes
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... NRP1(8829)

Description

Description générale

Chemical structure: peptide

Defensins (α and β) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins which include neutrophil peptides-1, -2 and -3, (NP-1, NP-2 and NP-3) are distinguished from the β-defensins by the pairing of their three disulfide bonds. Neutrophil-1 (NP-1) is expressed as the C-terminal portion of an inactive precursor protein, which also contains a 19 amino acid N-terminal signal sequence and a 45 amino acid polypeptide. The protein contains a six-cysteine motif that forms three intra-molecular disulfide bonds. It is a transmembrane glycoprotein. Recombinant human NP-1 is a 3.4kDa protein containing 30 amino acid residues.

Actions biochimiques/physiologiques

Neutrophil peptide-1 (NP-1) exhibits antimicrobial activity. It also has a chemotactic function in dendritic cells. It has a role in the growth and metastasis of cancer cells. The protein acts as multifunctional co-receptor and interacts with various signaling cascades. NP-1 associates with epithelial growth factor (EGF) and its receptor (EGFR). It also interacts with hepatocyte growth factor (HGF) and its receptor c-Met.

Séquence

ACYCRIPACI AGERRYGYCI YQGRLWAFCC

Forme physique

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable