SRP0239
EGFR Active human
recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)
Synonyme(s) :
ERBB1, Epidermal growth factor receptor, HER1, PIG61, Proto-oncogene c-ErbB-1
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Produit recombinant
expressed in baculovirus infected insect cells
Pureté
≥70% (SDS-PAGE)
Forme
aqueous solution
Puissance
≥0.14 units/mg
Poids mol.
87.3 kDa
Conditionnement
pkg of 10 μg
Concentration
>0.02 mg/mL
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... EGFR(1956)
Description générale
Epidermal growth factor receptor (EGFR) is a transmembrane protein and a member of a family of receptor tyrosine kinases (RTK) called avian erythroblastosis oncogene (BErbB). It contains a ligand-binding domain in its extracellular region, one transmembrane domain, and a tyrosine kinase-containing domain in its cytosolic region. EGFR is encoded by the gene mapped to human chromosome 7p11.2.
Application
EGFR Active human has been used in:
- photo-cross-linking assays
- StavroX cross-linking site identification
- in vitro kinase assay
Actions biochimiques/physiologiques
Epidermal growth factor receptor (EGFR) functions as a receptor for EGF-family of proteins, which are peptide growth factors. EGFR and ligand play a crucial role early embryonic development and in the renewal of stem cells in tissues such as the gut, liver and skin. This receptor-ligand system is also involved in the pathogenesis and progression of various types of carcinomas. In cancer, EGFR pathways promotes cellular proliferation, angiogenesis, metastasis and apoptosis. The microRNA miR-218-5p interacts with and controls EGFR, and in non-small cell lung cancer (NSCLC), the levels of EGFR are inversely related to miR-218-5p. miR-218-5p inhibits proliferation and migration of lung cancer cells by binding with EGFR, and thus, has potential in future NSCLC therapies.
Définition de l'unité
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.
Forme physique
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM glutathione and 3 mM DTT.
Notes préparatoires
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Marites Pasuelo Melancon et al.
ACS nano, 8(5), 4530-4538 (2014-04-24)
The purpose of this study was to compare the binding affinity and selective targeting of aptamer- and antibody-coated hollow gold nanospheres (HAuNS) targeted to epidermal growth factor receptors (EGFR). EGFR-targeting aptamers were conjugated to HAuNS (apt-HAuNS) by attaching a thiol-terminated
Julie K L Sinclair et al.
Biochemistry, 60(7), 500-512 (2021-02-10)
We previously reported a family of hydrocarbon-stapled peptides designed to interact with the epidermal growth factor receptor (EGFR) juxtamembrane (JM) segment, blocking its ability to form a coiled coil dimer that is essential for receptor activation. These hydrocarbon-stapled peptides, most
Filippo Lococo et al.
International journal of molecular sciences, 16(8), 19612-19630 (2015-08-22)
Assessment of biological diagnostic factors providing clinically-relevant information to guide physician decision-making are still needed for diseases with poor outcomes, such as non-small cell lung cancer (NSCLC). Epidermal growth factor receptor (EGFR) is a promising molecule in the clinical management
A novel approach in the treatment of cancer: targeting the epidermal growth factor receptor.
Ciardiello F, Tortora G
Clinical Cancer Research, 7(10), 2958-2970 (2001)
Tumor-suppressive miR-218-5p inhibits cancer cell proliferation and migration via EGFR in non-small cell lung cancer.
Zhu K, et al.
Oncotarget, 7(19), 28075-28085 (2016)
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