SRP0234

PI3 kinase [p110a(E545K)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

• Synonyme(s) : PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥55% (SDS-PAGE)
• Forme: aqueous solution
• Activité spécifique: ≥800 pmol/min-μg
• Poids mol.: 129 kDa; 88 kDa
• Conditionnement: pkg of 20 μg
• Concentration: 0.56 mg/mL
• Numéro d'accès NCBI: U79143 (p110alpha); XM_043865 (p85alpha)
• Numéro d'accès UniProt: P42336
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PIK3CA(5290)

Description

Description générale

Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1