SRP0229

WWP1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥15% (SDS-PAGE)

• Synonyme(s) : AIP5, Atrophin-1 interacting protein 5, NEDD4-like E3 ubiquitin-protein ligase, TGIF-interacting ubiquitin ligase 1 (Tiul1), WW domain containing E3 ubiquitin protein ligase 1
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥15% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 106 kDa
• Conditionnement: pkg of 20 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_007013
• Numéro d'accès UniProt: Q9H0M0
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... WWP1(11059)

Description

Description générale

Human WWP1 (GenBank Accession No. NM_007013), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.