SRP0130

EZH1/EED/SUZ12  human

recombinant, expressed in baculovirus infected insect cells

• Synonyme(s) : ENX-2, EZH1, Enhancer of zeste homolog 1, KMT6B, Lysine N-methyltransferase 6B
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥80% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 51 kDa (EED); 86 kDa (EZH1); 87 kDa (SUZ12)
• Conditionnement: pkg of 50 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_001991 (EZH1); NM_003797 (EED); NM_015355 (SUZ12)
• Numéro d'accès UniProt: Q92800
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... EZH1(2145)

Description

Description générale

Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12,. NM_015355, amino acids 2-end, with N-terminal His tag.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.