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SH2421

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

p53 Pathway

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Niveau de qualité

200

Gamme de produits

MISSION®

Température de stockage

−20°C

Informations sur le gène

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Description générale

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Autres remarques

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 242, Number of Clones: 1865
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Informations légales

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Alejo Efeyan et al.
Cell cycle (Georgetown, Tex.), 6(9), 1006-1010 (2007-04-26)
The process of malignant transformation universally entails genetic damage and oncogenic signaling, two stresses that are signaled to p53 through different genetic pathways. Based on this, it is possible to distinguish two jobs for p53: "guardian of the genome" that
Indrani Bose et al.
Journal of biosciences, 32(5), 991-997 (2007-10-05)
The p53 protein is well-known for its tumour suppressor function. The p53-MDM2 negative feedback loop constitutes the core module of a network of regulatory interactions activated under cellular stress. In normal cells, the level of p53 proteins is kept low

Articles

Oncogenes and Tumor Suppressors Reprogram Metabolism

Sigma-Aldrich presents an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.

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