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SEQR

Sigma-Aldrich

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Synonyme(s) :

WGA kit, Whole transcriptome amplification

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.55

Niveau de qualité

200

Technique(s)

whole genome amplification: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Caractéristiques et avantages

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Autres remarques

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Informations légales

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

T3038

Trizma® hydrochloride solution, 1 M, BioReagent, for molecular biology

S9430

Colorant SYBR® Green I pour acides nucléiques sur gel, 10,000 × in DMSO

W4502

Eau, Nuclease-Free Water, for Molecular Biology

AMPD1

DNase I, Amplification Grade

S4438

JumpStart™ Taq ReadyMix avec SYBR® Green™, for quantitative PCR, MgCI2 in buffer

NA1020

GenElute™ PCR Clean-Up Kit, sufficient for 70 purifications

WTA1

TransPlex® Whole Transcriptome Amplification Kit, DNA polymerase separate.

WTA2

TransPlex® Complete Whole Transcriptom Amplification Kit, DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Cecilia Lindestam Arlehamn et al.
Journal of immunology (Baltimore, Md. : 1950), 193(6), 2931-2940 (2014-08-06)
In latent tuberculosis infection (LTBI) spread of the bacteria is contained by a persistent immune response, which includes CD4(+) T cells as important contributors. In this study we show that TB-specific CD4(+) T cells have a characteristic chemokine expression signature

Protocoles

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

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