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Key Documents

SAB5201915

Sigma-Aldrich

Monoclonal Anti-ERp57-Atto 390 antibody produced in mouse

clone 4F9, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-PDIA3

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.45

Source biologique

mouse

Conjugué

Atto 390 conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

4F9
monoclonal

Description

target molecular weight: 57 kDa

Forme

buffered aqueous solution

Espèces réactives

human (Tested)

Conditionnement

pkg of 100 μg

Concentration

1.0 mg/mL

Technique(s)

immunofluorescence: 1:100
western blot: 1:1000

Isotype

IgG2b

Numéro d'accès Protein ID

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PDIA3(2923)

Immunogène

Immunogen type: Recombinant protein
Immunogen Species: Human
Immunogen Region: See Notes section/Other
Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

Application


Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa

Description de la cible

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .

Forme physique

50% glycerol, 0.09% Sodium Azide
PBS pH 7.4

Notes préparatoires

Derived from cell/tissue culture

Stockage et stabilité

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Autres remarques

Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase

Other:
Immunogen region: Amino acids 25-505
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2


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