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Key Documents

SAB4200818

Sigma-Aldrich

Anti- Proteus mirabilis antibody produced in rabbit

IgG fraction of antiserum

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Forme d'anticorps

IgG fraction of antiserum

Clone

polyclonal

Description

Research area: Microbiome

Forme

buffered aqueous solution

Poids mol.

~70 kDa

Espèces réactives

Proteus mirabilis

Conditionnement

antibody small pack of 25 μL

Concentration

~1 mg/mL

Technique(s)

immunoblotting: 1:10,000-1:20,000 using Proteus mirabilis LPS
indirect ELISA: 1:16,000-1:32,000

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Catégories apparentées

Description générale

Proteus mirabilis is a Gram negative rod-shaped bacteria, belongs to the Enterobacteriaceae family. Member of the Proteus genus (Proteus spp.) which also includes Proteus mirabilis, Proteus penneri and Proteus hauseri, originally characterize by their ability to swarm on solid surfaces, are widespread in the environment and the gastrointestinal tract of human and animals and known to be an opportunistic pathogens isolated from urine, wounds and other clinical sources. The Proteus spp. bacteria, are distinguished by their reactions for indole production, salicin fermentation and aesculin hydrolysis. P. vulgaris produces indole which differentiates it from the indole-negative P. mirabilis and P. penneri. Proteus spp. bacteria may also be found in soil or water habitats where they often regarded as indicators of fecal pollution and a contamination threat for potential water or seafood poisoning.

Immunogène

Proteus mirabilis OXK dead bacteria, ATCC strain 15146

Application

Anti-Proteus mirabilis antibody recognizes P. mirabilis whole extract and P. mirabilis LPS, the antibody also recognizes an additional ~70kDa band suspected as bacterial HSP70 (DNAK) in whole extract P. vulgaris, P. gingivalis, E.coli K-12, P.aeruginosa, S. flexneri, S. enterica and E. faecalis but it has no cross reactivity with P. vulgaris LPS. The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA. 

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Autres remarques

This product is for R&D use only, not for drug, household, or other uses.

Clause de non-responsabilité

This product is for R&D use only, not for drug, household, or other uses.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Jessica N Schaffer et al.
Microbiology spectrum, 3(5) (2015-11-07)
Proteus mirabilis is a Gram-negative bacterium and is well known for its ability to robustly swarm across surfaces in a striking bulls'-eye pattern. Clinically, this organism is most frequently a pathogen of the urinary tract, particularly in patients undergoing long-term
Noriyuki Nagano et al.
Journal of clinical microbiology, 41(12), 5530-5536 (2003-12-10)
Nineteen multidrug-resistant Proteus mirabilis strains were isolated from 19 patients suffering from infections probably caused by P. mirabilis. These strains were recovered from urine or other urogenital specimens of 16 inpatients and three outpatients with a hospitalization history in a
ANTIMICROBIAL RESISTANCE PATTERNS OF PROTEUS ISOLATES FROM CLINICAL SPECIMENS
Bahashwan, et al.
EUROPEAN SCIENTIFIC JOURNAL, 9, 188-202 (2013)
C M O'Hara et al.
International journal of systematic and evolutionary microbiology, 50 Pt 5, 1869-1875 (2000-10-18)
Strains traditionally identified as Proteus vulgaris formed three biogroups. Biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as Proteus penneri. Biogroup 2, characterized by positive reactions for indole, salicin and aesculin
N Pal et al.
Annals of medical and health sciences research, 6(5), 267-273 (2017-05-16)
Proteus species cause a variety of community- and hospital-acquired illnesses. Synthesis of β-lactamases is the predominant mechanism for resistance to β-lactam antibiotics. Among the β-lactamases, extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases are the most common. The objective of this

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