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Key Documents

SAB4200287

Sigma-Aldrich

Anti-Actin, Muscle specific antibody, Mouse monoclonal

clone HHF-35, purified from hybridoma cell culture

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

HHF-35, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~42 kDa

Espèces réactives

rat, bovine, canine, chicken, human, mouse, hamster, monkey

Concentration

~1.0 mg/mL

Technique(s)

immunocytochemistry: 2-4 μg/mL using A10 cells
immunohistochemistry: suitable
western blot: 0.1-0.2 μg/mL using A10 cell extracts

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ACTB(60)

Description générale

Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis.

Immunogène

SDS-extracted protein fraction of human myocardium.

Application

The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry.

Actions biochimiques/physiologiques

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Live cell imaging of the cytoskeleton
Test, 505, 203-217 (2012)
Live cell imaging of the cytoskeleton
Methods in Enzymology, 505, 203-217 (2012)
T Tsukada et al.
The American journal of pathology, 126(1), 51-60 (1987-01-01)
A monoclonal antibody to muscle cell actin isotypes was produced and characterized. Immunocytochemical analysis of methanol-Carnoy's-fixed, paraffin-embedded human tissue revealed that this antibody, termed HHF35, reacts with skeletal muscle cells, cardiac muscle cells, smooth muscle cells, pericytes, and myoepithelial cells
Actin structure and function
Dominguez R, et al.
Annual Review of Biophysics, 40, 169-186 (2011)

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