SAB4200248
Anti-Actin antibody, Mouse monoclonal
clone MM2/193, purified from hybridoma cell culture
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About This Item
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
MM2/193, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~42 kDa
Espèces réactives
mouse, human, rat, canine, monkey
Concentration
~1.0 mg/mL
Technique(s)
western blot: 1-2 μg/mL using HeLa cell extracts
Isotype
IgM
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ACTB(60)
Description générale
Monoclonal anti-actin (mouse IgM isotype) is derived from the hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes. Monoclonal anti-actin recognizes human, monkey, dog, rat and mouse actin (not tested in other species).
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
In mammals, actin protein consists of six different isoforms encoded by separate genes. The four isoforms αskeletal-actin, α cardiac-actin, αsmooth-actin and γsmooth-actin are expressed in skeletal, cardiac and smooth muscle. The other two isoforms, β cyto-actin and γ cyto-actin are ubiquitously expressed.
Immunogène
hybridoma MM2/193 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes
Application
Monoclonal Anti-Actin antibody produced in mouse has been used in immunoblotting.
Actions biochimiques/physiologiques
Actin plays a vital role in various cellular processes, such as cell division, migration, junction formation, chromatin remodeling, transcriptional regulation, vesicle trafficking and cell shape regulation. Polymorphism in the smooth muscle alpha-actin (ACTA2) is associated with the development of thoracic aortic aneurysms and dissections.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Stockage et stabilité
Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Autres remarques
In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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