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SAB4200100

Sigma-Aldrich

Anti-ATG13 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

ATG13 Antibody - Anti-ATG13 antibody produced in rabbit, Atg13 Antibody, Anti-Autophagy-related protein 13

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~65 kDa

Espèces réactives

human, rat, mouse

Concentration

~1.0 mg/mL

Technique(s)

western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells.

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KIAA0652(9776)

Description générale

Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2).

Spécificité

Anti-Atg13 recognizes human, mouse, and rat Atg13.

Application

Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Actions biochimiques/physiologiques

Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains
Nishimura T, et al.
The Embo Journal, 36(12), 1719-1735 (2017)
Di Ge et al.
Autophagy, 10(6), 957-971 (2014-06-01)
MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. No chemical activators of MTOR have been found, and the urgent challenge
Selective Autophagy of Mitochondria on a Ubiquitin-Endoplasmic-Reticulum Platform
Zachari M, et al.
Developmental Cell (2019)
Systematic analysis of ATG13 domain requirements for autophagy induction
Wallot-Hieke N, et al.
Autophagy, 14(5), 743?763-743?763 (2018)
Physical and neurobehavioral determinants of reproductive onset and success
Day FR, et al.
Nature Genetics, 48(6), 617-617 (2016)
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