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Merck
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Principaux documents

SAB2500963

Sigma-Aldrich

Anti-α-Smooth Muscle Actin (ACTA2) Antibody,

goat polyclonal

Synonyme(s) :

Anti-ACTA2, Anti-OTTHUMP00000020042

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Nom du produit

Anti-Smooth Muscle α-Actin antibody produced in goat, affinity isolated antibody, buffered aqueous solution

Source biologique

goat

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

human, mouse, rat

Technique(s)

ELISA: suitable
western blot: suitable

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ACTA2(59)

Description générale

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). Difficulties have been encountered in producing polyclonal antibodies due to the highly conserved nature of actin.
Goat polyclonal anti-smooth muscle α-Actin antibody reacts with canine, human, mouse, and rat smooth muscle α-actins (ACTA2).

Immunogène

Peptide with sequence EEEDSTALVC from the N Terminus of the protein sequence according to NP_001604.1.

Application

Goat polyclonal anti-smooth muscle α-Actin antibody is used to tag smooth muscle α-actin (ACTA2) for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of smooth muscle α-actin in smooth muscle structure and as a marker of myofibroblast formation.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Xia Wang et al.
Bio-protocol, 7(17) (2017-10-06)
Cardiac neural crest cells (CNCCs) originate at the dorsal edge of the neural tube between the otic pit and the caudal edge of the 3rd somite, and migrate into the pharyngeal arches and the heart. We have shown that fibronectin
Rebecca M Shaffer et al.
American journal of obstetrics and gynecology, 221(4), 330-330 (2019-05-19)
The use of polypropylene prolapse mesh to treat pelvic organ prolapse has been limited by mesh-related complications. Gynemesh PS mesh, implanted via sacrocolpopexy in rhesus macaques, had a negative impact on the vagina with thinning of vaginal muscularis and decreased
Riccardo Coletta et al.
Journal of tissue engineering and regenerative medicine, 10(2), 178-182 (2015-08-04)
The ability to grow embryonic organs ex vivo provides an opportunity to follow their differentiation in a controlled environment, with resulting insights into normal development. Additionally, similar strategies can be used to assess effects on organogenesis of physical and chemical
Xi Zeng et al.
Experimental and therapeutic medicine, 15(1), 1062-1068 (2018-02-13)
The aim of the present study was to elucidate the effect of transmembrane protein 16A (TMEM16A) on portal vein smooth muscle cell (PVSMC) proliferation associated with portal vein remodeling in portal hypertension (PHT). Sprague-Dawley rats were subjected to bile duct
Jin-Yun Tan et al.
Molecular medicine reports, 14(5), 4445-4453 (2016-10-26)
Rab5a, a key member of the Rab family of GTPases, was determined to be a regulator of vascular smooth muscle cell (VSMC) proliferation and migration. However, the exact regulatory mechanism remains unclear. As Rab5a has been shown to be associated

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