S8140

SSPE Buffer

for Northern and Southern blotting, powder blend

• Code UNSPSC : 41105319
• Nomenclature NACRES : NA.25

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Forme: powder blend
• pH: 7.2-7.6 (20 × in water, high purity, ambient)
• Solubilité: water, high purity: 20 ×, clear, colorless
• Activité étrangère: DNAse, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; RNAse, none detected
• Température de stockage: room temp

Description

Description générale

SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.

Application

• Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).


• Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).

Caractéristiques et avantages

• Easy to reconstitute powder
• Multi-purpose use in hybridizations

Composants

Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges