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P7181

Sigma-Aldrich

Polyethylene glycol solution

Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

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About This Item

Numéro MDL:
MFCD00081839
Code UNSPSC :
12161902
ID de substance PubChem :
24898829

Qualité

Hybri-Max™

Niveau de qualité

200

Stérilité

0.2 μm filtered

Gamme de produits

BioReagent

Forme

solution

Poids mol.

average mol wt 1,450

Concentration

50 % (w/v)

Technique(s)

cell culture | hybridoma: suitable

Impuretés

endotoxin, tested

Température de stockage

2-8°C

Chaîne SMILES 

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

Clé InChI

LYCAIKOWRPUZTN-UHFFFAOYSA-N

Catégories apparentées

Description générale

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.

Application

Polyethylene glycol solution has been used in the hybridization of the following:
  • irradiated camel fibroblast cells with A23 hamster cells
  • mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
  • rabbit splenocytes and cell line 240E-1

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Actions biochimiques/physiologiques

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.

Conditionnement

Packaged in sealed ampules under nitrogen.

Autres remarques

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Reconstitution

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Informations légales

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

212.0 °F - closed cup

Point d'éclair (°C)

100 °C - closed cup

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Fatima Amanat et al.
mBio, 12(4), e0100221-e0100221 (2021-07-28)
After first emerging in late 2019 in China, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has since caused a pandemic leading to millions of infections and deaths worldwide. Vaccines have been developed and authorized, but the supply of these vaccines
Zhengrong Zhang et al.
Cell death and differentiation, 28(9), 2765-2777 (2021-04-22)
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus is highly contagious and causes lymphocytopenia, but the underlying mechanisms are poorly understood. We demonstrate here that heterotypic cell-in-cell structures with lymphocytes inside multinucleate syncytia are prevalent in the lung tissues
Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000 RAD and 15000 RAD
Perelman PL, et al.
Scientific reports, 8(1), 1982-1982 (2018)
Retinol encapsulated into amorphous Ca2+ polyphosphate nanospheres acts synergistically in MC3T3-E1 cells
Muller WEG, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 93(2), 214-223 (2015)
Development of Monoclonal Antibodies against CMP-N-Acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase 1 (ST3Gal-I) Recombinant Protein Expressed in E. coli
Gupta AK, et al.
Biochemistry Research International, 2015(1), 1982-1982 (2015)
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