P1860

Cocktail d'inhibiteurs de protéases

for use in tissue culture media, DMSO solution

• Synonyme(s) : Protease Inhibitor Solution, protease inhibitor
• Numéro CE : 200-664-3
• Numéro MDL: MFCD00677817
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 200
• Forme: DMSO solution
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Spécificité

Inhibe les protéases à sérine, à cystéine et à acide aspartique, ainsi que les aminopeptidases.

Application

Protease Inhibitor Cocktail has been used:

• as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
• to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
• in trypsin solution for trypsin-inhibited studies
• as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Actions biochimiques/physiologiques

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

Caractéristiques et avantages

• Broad specificity
• Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
• Contains no metal chelators: Ensures compatibility with downstream applications
• Convenient packaging: 1 mL in glass bottle
• Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Composants

Aprotinine
Bestatine
E-64
Leupeptine
Pepstatine A

Attention

Après une exposition de 48 heures, ce produit s'est avéré non toxique envers les lignées cellulaires adhérentes A431, CHO, COS, HepG2 et HeLa, ainsi que les lignées cellulaires en suspension Jurkat et HL-60.

Quantité

Utiliser à une dilution au 1/200e ou plus dans du milieu de culture cellulaire, afin d'éviter la dégradation protéolytique des protéines sécrétées

Forme physique

Solution dans du DMSO (D 2650, Hybri-Max).

Autres remarques

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): 188.6 °F
• Point d'éclair (°C): 87 °C