Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

Autres documents

P1167

Sigma-Aldrich

Pituitary Extract bovine

lyophilized powder, suitable for cell culture

Synonyme(s) :

BPE, PEX

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro MDL:
MFCD00163156
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75

Source biologique

bovine

Niveau de qualité

200

Forme

lyophilized powder

Puissance

15 - 200 μg/mL EC50

Qualité

endotoxin tested

Conditionnement

pkg of 5 mg

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤50 EU/mg

Couleur

brown

Solubilité

0.85% sodium chloride: soluble 1 vial/mL, clear to hazy, faintly yellow to dark yellow-orange

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Pituitary Extract, bovine (PEX) is an extract of bovine pituitary tissue containing growth factors.

Application

Pituitary Extract bovine has been used as a component in the Roswell Park memorial institute 1640 (RPMI-1640) to culture Rb-/- epithelial cells. It has also been used as a component in the Dulbecco′s modified Eagle′s medium (DMEM) for culturing Schwann cells.

Actions biochimiques/physiologiques

Pituitary Extract, bovine (PEX) or BPE is essential for mitotic activity and promotes the growth of several types of mammalian cells. It favors the proliferation of keratocytes culture and bovine knee chondrocytes. BPE helps in the synthesis of collagen and glycosaminoglycans (GAGs) in bovine knee chondrocytes.

Forme physique

Lyophilized from sodium phosphate buffer

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Simon W Hayward et al.
Methods in molecular biology (Clifton, N.J.), 218, 17-33 (2003-03-06)
The ability to rescue viable prostate precursor tissue from Rb-/-fetal mice has allowed for the generation of Rb-/-prostate tissue and Rb-/-prostate epithelial cell lines. Herein, we provide a protocol for the rescue of urogenital precursor tissue from mouse embryos harboring
Yung-Chih Kuo et al.
Biotechnology progress, 21(6), 1708-1715 (2005-12-03)
In vitro cultivation of primary bovine knee chondrocytes (BKCs), using bovine pituitary extract (BPE) and porous scaffolds composed of polyglycolide (PGA) and 85/15 poly(lactide-co-glycolide) (PLGA), was investigated. Here, BPE was prepared from fresh bovine pituitaries, and cylindrical PGA/PLGA scaffolds with
J C Willey et al.
Journal of cellular physiology, 124(2), 207-212 (1985-08-01)
The mechanisms of action of extracellular mitogens for normal human bronchial epithelial cells (NHBE) were investigated by observing their effects on selected biochemical pathways when the cells were incubated in serum-free media. We find that (a) epidermal growth factor (EGF)
S L Hammond et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(17), 5435-5439 (1984-09-01)
A serum-free medium with bovine pituitary extract as the only undefined supplement has been developed for long-term culture of human mammary epithelial cells. This medium supports serial subculture of normal cells for 10-20 passages (1:10 splits) without conditioning or special
Jie Liu et al.
Animal reproduction science, 115(1-4), 49-57 (2008-12-26)
Different culture systems were evaluated for their ability to support attachment and proliferation of the somatic cells obtained from ovine semen. Ejaculates (n=14) were collected from eight rams representing three breeds, Dorper, Suffolk and Hampshire. All samples were processed immediately
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique