OGS1879

PSF-TEF1-NH2-INTS - INVERTASE SECRETION PLASMID

plasmid vector for molecular cloning

• Synonyme(s) : cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.85

Propriétés

• Forme: buffered aqueous solution
• Poids mol.: size 6787 bp
• Sélection de bactéries: kanamycin
• Origine de la réplication: 2Micron; pUC (500 copies)
• Clivage des peptides: no cleavage
• Localisation du marqueur peptidique: N-terminal
• Promoteur: Promoter name: TEF1; Promoter activity: constitutive; Promoter type: yeast
• Gène reporter: none
• Signal de sécrétion: alpha factor FL
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• Sélection de levures: uracil

Description

Description générale

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.

About the Peptide Tag:This plasmid contains an Invertase (Ints) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.

Séquence

To view sequence information for this product, please visit the product page

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable