OGS1658

PSF-TEF1-NH2-FLAG^®-6HIS-EKT - N-TERMINAL FLAG^® AND 6 HIS DUAL TAG YEAST PLASMID

plasmid vector for molecular cloning

• Synonyme(s) : cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.85

Propriétés

• Étiquette/Marqueur: 6-His tagged; FLAG^® tagged
• Forme: buffered aqueous solution
• Poids mol.: size 6772 bp
• Sélection de bactéries: kanamycin
• Origine de la réplication: 2Micron; pUC (500 copies)
• Clivage des peptides: EKT
• Localisation du marqueur peptidique: N-terminal
• Promoteur: Promoter name: TEF1; Promoter activity: constitutive; Promoter type: yeast
• Gène reporter: none
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.

About the Peptide Tag:This plasmid contains an n-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH. This plasmid also contains a secondary Flag protein tag. The sequence of this tag is: DYKDDDDKWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.

About the Cleavage Tag: This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a EKT cleavage tag. The protein sequence of the cleavage tag is: DDDDK. Enterokinase (EKT) protease cleaves after the Lysine residue. It can cleave at other basic residues but this is dependent on protein confirmation. If a proline follows the site it will not cut. None of our products contain a proline after the site.

Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.

Séquence

Quick-reference Plasmid Map

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

Autres remarques

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics^™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

Informations légales

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Oxford Genetics is a trademark of Oxford Genetics Ltd