N5538

Monoclonal Anti-3-Nitrotyrosine antibody produced in mouse

clone 18G4, purified immunoglobulin, buffered aqueous glycerol solution

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 18G4, monoclonal
• Forme: buffered aqueous glycerol solution
• Poids mol.: antigen ~55 kDa
• Espèces réactives: wide range
• Technique(s): western blot: 1:1,000 using mouse and rat brain
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: nitration (Tyr)
• Informations sur le gène: human ... NOS1(4842)

Description

Description générale

3-nitrotyrosine (3-NT) is synthesized by the incorporation of nitro triatomic group (-NO₂) at the third position of the phenolic ring in the tyrosine residue, in response to the production of peroxynitrite in the oxidative and nitrosative stress.

Immunogène

3-Nitrotyrosine-KLH.

Application

Monoclonal Anti-3-Nitrotyrosine antibody produced in mouse has been used in western blot analysis and immunohistochemistry.

Actions biochimiques/physiologiques

3-Nitrotyrosine (3-NT) in tissue extracts is a marker for the determination of protein damage induced by peroxynitrite. 3-NT is used as a biomarker to determine the protein nitration, associated with various disorders. It is implicated in diseases characterized by endothelial dysfunction as in stroke, myocardial infarction, atherosclerosis and diabetes.

Description de la cible

Monoclonal Anti-3-Nitrotyrosine recognizes 3-nitrotyrosine in most species. Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling.

Forme physique

Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, and 3 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable