N5288

Anti-NQO1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-DIA4, Anti-DT-diaphorase, Anti-Diaphorase 4, Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1, Anti-NAD(P)H dehydrogenase, quinone 1, Anti-NMOR1
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~28 kDa
• Espèces réactives: mouse, rat, human
• Concentration: ~1 mg/mL
• Technique(s): immunoprecipitation (IP): 2-5 μg using whole extract of human HeLa cells; indirect immunofluorescence: 2-5 μg/mL using human HeLa cells; western blot: 0.1-0.2 μg/mL using whole extract of rat NRK cells; western blot: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells
• Numéro d'accès UniProt: P15559
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NQO1(1728) ; mouse ... Nqo1(18104) ; rat ... Nqo1(24314)

Description

Description générale

NADPH:quinone oxidoreductase (NQO1) is an FAD containing electron reductase that regulates the detoxification of quinones obtained from the phenolic metabolites of benzene. It is a homodimeric protein that can bind either of the reducing cofactors; NADPH or NADH . NQO1 regulates chemoprotection, neuroprotection, antitumor quinones and antioxidative defense mechanisms . NQO1 may be overexpressed in breast, colon, pulmonary and hepatic tumors.

NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) is a 2-electron reductase. NQO1 is a FAD-containing protein that exists as a homodimer. It is ubiquitously expressed.

Spécificité

Anti-NQO1 (C-terminal) antibody is specific for rat, mouse, and human NQO1. The immunizing peptide specifically inhibits the detection of NQO1 band by immunoblotting.

Immunogène

Synthetic peptide sequence corresponding to amino acid residues 261-274 of human NQO1, with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Anti-NQO1 (C-terminal) antibody may be used in immunological applications such as immunoprecipitation, immunofluorescence and immunoblotting (approximately 28 kDa).

Anti-NQO1 (C-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunoprecipitation
• immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

NADPH:quinone oxidoreductase (NQO1, DTdiaphorase) can bioactivate certain antitumor quinones. NQO1 has a role in antioxidant defense via the generation of antioxidant forms of ubiquinone and vitamin E. High levels of NQO1 gene expression has been observed in liver and lung tumors as compared to normal tissues of the same origin.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)